RNA-seq of adult Opisthorchis felineus: 50 mkg/kg PZQ - SRA

SRX3342142: RNA-seq of adult Opisthorchis felineus: 50 mkg/kg PZQ
1 ILLUMINA (Illumina HiSeq 2000) run: 48.4M spots, 4.3G bases, 2.6Gb downloads

Design: Samples of 50 adults were homogenized in liquid nitrogen and RNA was extracted using the RNEasy Mini (Qiagen) kit. cDNA libraries were then prepared using 250–500 ng of total RNA. Briefly, mRNA were purified using oligo-dT primer coated magnetic beads (Invitrogen). Double stranded cDNA was synthesized using SuperscriptII reverse transcriptase (Invitrogen) and random hexamer primer (Invitrogen) and purified using the Ampure XP kit (Invitrogen). Illumina sequencing adapters were then ligated to the cDNA. The resulting PCR product was then purified with magnetic beads (Invitrogen), electrophoresed through a 2% agarose gel following excision of 300–500 nucleotide PCR products. The libraries were sequenced with paired-end protocol (2 ? 101 bp) using Illumina Hiseq2000 and TruSeq SBS chemistry. Demultiplexing was performed using CASAVA-1.8.2 (Illumina).

Submitted by: Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences

Study: Praziquantel effects on Opisthorchis felineus transcriptome

PRJNA416247 • SRP122880 • All experiments • All runs

show Abstracthide Abstract

Opisthorchis felineus (Rivolta, 1884) or European liver fluke is epidemiologically important parasite that infect mammals including humans. O. felineus is widespread on the territory of Russia, Kazakhstan, and European countries. Praziquantel is the drug of choice for the treatment of opisthorchiasis, but this drug effects on O. felineus are poorly studied.

Sample: Opisthorchis felineus adult worms treated by 50 mk/kg praziquantel in vivo

SAMN07945865 • SRS2643564 • All experiments • All runs

Organism: Opisthorchis felineus

Library:

Name: OF_adult_PZQ_treated_50 mkg_kg

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Runs: 1 run, 48.4M spots, 4.3G bases, 2.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR6233713	48,408,494	4.3G	2.6Gb	2018-11-01

ID:: 4672152

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